2.4. Preparation of CMC-β-CD Nanogel Loaded with CK
This protocol is extracted from research article:
Inhibitory Effect of pH-Responsive Nanogel Encapsulating Ginsenoside CK against Lung Cancer
Polymers (Basel), May 28, 2021; DOI: 10.3390/polym13111784

The CMC-β-CD/CK Ngs (CK-Ngs) were prepared via a water-in-oil inverse microemulsion method [22]. Firstly, Span 80 (9.33 mmol) and Tween 80 (0.31 mmol) were dissolved in 18 mL of cyclohexane. A certain amount of CK was dissolved in methanol, then added dropwise to 2 mL β-CD-CHO under probe ultrasound (ultrasound time is 2 min, working 2 s interval 1 s). The resulting mixed solution was added to cyclohexane and sonicated with the probe for 5 min to obtain a microemulsion. CMC-NH2 was continuously dripped into the above microemulsion, and then the resulted mixture stirred for 24 h at room temperature. After the stirring, the CK-Ngs were obtained by centrifuging (8000 rpm, 5 min) and washed with ethanol for 3 times to remove the excess surfactant. Finally, the CK-Ngs powders were obtained by freeze-drying. The synthesis process of the blank Ngs (CMC-β-CD Ngs) was as described above.

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