The Folin–Ciocalteu method was referred for the estimation of total phenols content (TPC) [44]. Samples of 10 µL prepared for assaying total phenols was mixed with 790 µL milli-Q water and 50 µL Folin–Ciocalteu reagent and vortexed. After the addition of 150 µL sodium carbonate solution (20%), the content was quantified at 750 nm after 1 h of incubation at room temp (23.5 °C). Total polyphenol was expressed as gallic acid equivalents (mg GAE L−1).

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