R v3.5.0 and SPSS v23.0.0.0 were employed for statistical analysis, and the GraphPad Prism v8.4.3 was used for scientific drawing. False discovery rate (FDR) ≤0.05 was designed as threshold for filtering the significantly difference between tumor and normal tissues in bioinformatics analysis, while a Student’s t-test was used for comparing the difference between the test and control groups in experimental validations. Meanwhile, Kaplan–Meier curves and Cox regression analysis with log-rank p test were applied into survival analysis, in which p <0.05 was considered as statistically significant.

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