2.7. Histological and Immunohistochemical Examination of Grafts
This protocol is extracted from research article:
Assessment of Electrospun Pellethane-Based Scaffolds for Vascular Tissue Engineering
Materials (Basel), Jul 1, 2021; DOI: 10.3390/ma14133678

Cross-sections of VGs (thickness of 10 µm) were obtained using a Microm HM-550 cryomicrotome (Carl Zeiss, Germany) and used for histological and immunohistochemical examination. Samples were stained with hematoxylin and eosin following a classic protocol (H&E; Biovitrum, St. Petersburg, Russia). A detailed protocol of immunohistochemical staining by primary monoclonal and polyclonal antibodies of type IV collagen (clone PHM-12 + CIV22), α-SMA (clone 1A4/asm-1), and factor VIII (rabbit polyclonal) conjugated with horseradish peroxidase was outlined in manuscript [26].

The thickness of neointima and calcified area were assessed quantitatively as previously described [27]. Calcified regions were determined as dark purple regions on H&E sections, as described in manuscript [28].

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.