The solutions for electrospinning were prepared using polymer (Pellethane 2363-80A (Pel-80A), Lubrizol Advanced Materials, Ritterhude, Germany), proteins (gelatin (Gel) and bivalirudin (Bv), Sigma, Missouri, MO, USA) and 1,1,1,3,3,3-hexafluoroisopropanol as a solvent in accordance to earlier described protocols [25]. To determine biocompatibility and hemocompatibility ex vivo, in vivo functioning electrospun scaffolds were produced using solutions of 3.5% Pel-80A, 3.5% Pel-80A + 10% Gel, 3.5% Pel-80A + 1.5% Bv or 3.5% Pel-80A + 10% Gel + 1.5% Bv.

The matrices (thickness = 150–160 µm) and vascular grafts (1.8 mm diameter, wall thickness = 130–150 µm) were fabricated using an NF-103 (MECC, Ogori-shi, Japan) electrospinning device under the following conditions: the feed rate 1–1.15 mL/h, capillary-collector distance 19–20 cm, voltage 18.5–24 kV, and rotation speed of drum collector 300 rpm.

The electrospun scaffolds were evacuated under forvacuum for 12 h to evaporate remaining solvent, packed with zip-lock polyethylene containers, sterilized with 25 kGy electron beam irradiation using an ILU-6 accelerator (Institute of Nuclear Physics, SB RAS), and stored at 4 °C.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.