Inhibition of SARS-CoV-2 in Vero E6 Cells
This protocol is extracted from research article:
Inhibition of Human Coronaviruses by Antimalarial Peroxides
ACS Infect Dis, Mar 30, 2021; DOI: 10.1021/acsinfecdis.1c00053

An end point titration assay for TCID/50 PFU determinations was used for SARS-CoV-2 inhibition in Vero E6. Vero E6 monolayers in 96 well plates were infected at an MOI of 0.05 with SARS CoV-2 in DMEM + 2% FBS and incubated for 1 h at 37 °C, 5% CO2. Inoculum was aspirated, and cells were rinsed once with DMEM (100 μL) without FBS. Serial 2-fold dilutions of compounds in DMEM 2% FBS were added to duplicate wells and incubated for 72 h at 37 °C, 5% CO2. Monolayers were stained with 2% crystal violet in a 20% methanol solution.

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