Formalin-fixed paraffin-embedded tissue (FFPE) sections from archival tonsil tissue and the breast and NSCLC TMAs were cut in 4 µm serial sections onto positively charged slides. The NSCLC TMA block consisted of 144 cores, and the breast cancer TMA block contained 168 cores. Each core was 0.6 mm in diameter and represented an individual patient. Three of the cores on each of the two TMAs were used as on-slide controls for setting thresholds of PD-L1 positivity. TMAs were supplied by Yale Pathology Tissue Services (New Haven, Connecticut, USA). Each of the six study sites received 10 tonsil slides, two slides from the breast cancer TMAs, and two slides from the NSCLC TMAs. For a detailed description of tissue section serialization and distribution, please see online supplemental table 1.


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