RIP assay was carried out with a Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Billerica, MA, USA) according to the manufacturer’s instructions. Cells were lysed in the presence of protease and RNase inhibitors. Magnetic beads were then pre-incubated with IgG (Santa Cruz Biotechnology, sc-2025) or anti-E2F1 (Santa Cruz Biotechnology, sc-251). The prepared cell lysates underwent immunoprecipitation at 4°C overnight, and the co-precipitated RNAs were detected by qRT-PCR.

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