Cells were lysed in radio immunoprecipitation assay buffer (KeyGen, Nanjing, China) containing protease inhibitor PMSF (KeyGen, Nanjing, China) and analyzed for total protein concentration. Western blotting was obtained utilizing 20–40 μg of lysate protein according to the standard protocol. The following antibodies were used in this study: GLUT1 (Abcam, ab652, 1:1,000 dilution), GLUT2 (Abcam, ab54460, 1:1,000 dilution), GLUT3 (Abcam, ab41525, 1:1,000 dilution), GLUT4 (Abcam, ab654, 1:1,000 dilution), GLUT5 (Abcam, ab36057, 1:1,000 dilution), HK2 (Cell Signaling Technology, 2867, 1:1,000 dilution), ALDOC (Abcam, ab87122, 1:1,000 dilution), ENO1 (Proteintech, 11204-1-AP, 1:1,000 dilution), PKM (Abcam, ab38237, 1:1,000 dilution), LDH (Cell Signaling Technology, 3582, 1:1,000 dilution), ACTIN (Abcam, ab15265, 1:1,000 dilution), and E2F1 (Santa Cruz Biotechnology, sc-251, 1:1,000 dilution).

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