The procedures for animal care and use were approved by Nanjing Medical University Animal Care Committee. Ten female nude mice (4–6 weeks old) were purchased from Nanjing Medical University School of Medicine’s accredited animal facility and randomly divided into two groups. A549 cells stably expressing GAS6-AS1 or empty vector were injected subcutaneously in axilla of the mice. The volume of xenografts was measured every week with calipers ([length × width2]/2). All mice were euthanized 6 weeks after injection, and tumor xenografts were harvested and weighed.

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