Human GAS6-AS1, GLUT1, and E2F1 cDNA were PCR-amplified and then cloned into the expression vector pcDND3.1 (RiboBio, Guangzhou, China). Transfection was carried out with Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions, and empty vector plasmid was used as negative control. For experiments in vivo, GAS6-AS1 expression plasmids were packaged with pPACKH1 Lentivector Packaging Plasmid Mix (System Biosciences, Palo Alto, CA, USA) and were introduced into 293T cells. The liquid supernatant was transected into A549 cells, and stable transfected cells were positively selected with puromycin (Thermo Fisher Scientific, Shanghai, China) for 4–6 weeks.

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