Primary hippocampal neurons were obtained from C57BL/6J mouse embryos (E17.5–18.5) and maintained in Neurobasal-A medium with 2% B27 and 0.5 mM GlutaMAX-I (GIBCO) at 37°C and 5% CO2 (Dotti et al., 1988). For oligoIP experiments, neurons were transfected at DIV17-19.

Male mice, aged 2 to 4 months, were used for all experiments. Deletion of the GR from forebrain glutamatergic neurons was achieved by breeding GRlox/lox mice (Tronche et al., 1999) to Nex-Cre mice (Goebbels et al., 2006) to obtain GRGlu-Ctrl (GRlox/lox) and GRGlu-CKO (GRlox/lox:Nex-Cre) mice (Hartmann et al., 2017). Conditional MR mutant mice were obtained by breeding MRlox/lox mice to Camk2α–Cre mice (Berger et al., 2006; Minichiello et al., 1999) or Amigo2-Cre mice (McCann et al., 2021), respectively, to obtain Ctrl (MRlox/lox) and MRCamk2α-CKO (MRlox/lox:Camk2α-Cre) or Ctrl (MRlox/lox) and MRAmigo2-CKO (MRlox/lox:Amigo2-Cre) mice. For experiments in wild-type animals, C57BL/6J male mice were obtained from The Jackson Laboratory. All animals were kept under standard laboratory conditions and were maintained on a 12 h light–dark cycle (lights on from 07:00 am to 07:00 pm), with food and water provided ad libitum. All experiments conformed to National Institutes of Health guidelines and were carried out in accordance with the European Communities’ Council Directive 2010/63/EU and the McLean Hospital Institutional Animal Care and Use Committee. All efforts were made to minimize animal suffering during the experiments. The protocols were approved by the committee for the Care and Use of Laboratory animals of the Government of Upper Bavaria, Germany or by the local Institutional Animal Care and Use Committee, respectively.

Human microarray data were publicly available from the Allen Brain Institute (Hawrylycz et al., 2012). Log2 expression levels from donors (n = 6) were collected for FKBP5, NR3C1 and NR3C2 from each of the hippocampal subregions, CA1, CA2, CA3, CA4 and dentate gyrus (DG). See Table S1 for subject details.

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