Immediately following surgery, an experienced research nurse removed the seminal vesicles and color-labeled the specimen for laterality before it was placed in the +1 mm margin mold. The posterior part of the prostate was colored in yellow and the right and left sides were colored in green and red, respectively. If the mold could not be closed without compressing tissue, the mold with a +2 mm margin was used. Using the same PET/MRI scanner, high-resolution ex-vivo T2W imaging of the specimen was performed before formalin-fixation (<90 min post-resection). Transaxial, sagittal, and coronal localizers were acquired to aid in the positioning of the MRI slices (Fig. 2). All ex-vivo MRI slices were positioned with their center located over the outermost caudal side of the histology section (Fig. 2B). Increased MRI-signal originating from fluid in the slits, visible in the localizers, was used to guide the slice positioning. MRI-parameters are shown in Supplementary Table 1A.

Slice location – histopathology and ex-vivo MRI; A) Illustrates specimen sectioning using the slits in the prostate mold. B) The corresponding ex-vivo MRI slices, centered over the guiding slits in the mold. C) The resulting overlap between whole-mount section (pink), ex-vivo MRI slice (gray), and histopathology slice (darker pink). D) The ex-vivo MRI localizers, where the coronal and sagittal were used when positioning the ex-vivo MRI slices, guided by increased MRI signal in the slits in the mold (illustrated as spikes in the ellipsoids). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

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