Individually designed prostate molds were created for each patient to guide histopathology sectioning and to preserve the in-vivo shape and orientation of the specimen. A cubical shape was created in Fusion 360 (Autodesk, Inc. San Rafael, California, USA) (Fig. 1C). The cube had a standard volume of 6.6 × 6.6 × 6.6 cm3 and was constructed out of two separate parts that could be joined using a locking mechanism (Fig. 1D). Throughout the cube, eleven 1 mm thick slits were inserted (with 5 mm spacing), to be used for histopathology slicing. The exported RT-structs were converted into Standard Triangle Language (STL)-files using MICE toolkit (Nonpi Medical AB, Umeå, Sweden) [23]. The STL-files were imported into Meshmixer (Autodesk, Inc. San Rafael, California, USA) where they were smoothed and simplified by reducing the number of vertices (Fig. 1B) and subsequently used as inputs into Fusion 360 and subtracted from the cube. For each patient two molds were printed, with a margin of +1 mm and +2 mm, respectively, using MakerBot Replicator + 3D-printer (MakerBot Industries, Brooklyn, NY USA).

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