Constructed plasmids were transformed into Agrobacterium tumefaciens strain GV3101. Transformation of Arabidopsis was done following the vacuum infiltration method (Bechtold et al., 1993). Transformed T1 seedlings were selected with 0.1% glufosinate ammonium (Basta), followed by PCR and RT-PCR detection (Supplementary Table 7, primer#2). AtACT2 [actin 2 (A. thaliana); TAIR accession At3g18780] was used as internal control (primer #3). Leaves of positive lines were subjected to protoplasts extraction and YFP observation following a similar procedure as “maize leaf protoplasts extraction and GFP observation.”

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