Cells were lysed in 10 mM HEPES pH 7.3 NaCl 1% NP‐40 with protease inhibitor (Millipore Sigma #4693132001). Collected protein was denatured at 37°C for 30 minutes without addition of 2‐mercaptoethanol and 20 μg total protein lysate per lane was run on western blot. Primary antibody was rat anti‐GFP (Biolegend #338002) at 1:2000 and secondary goat anti‐rat IgG horseradish peroxidase (HRP) conjugated (Millipore Sigma #AB183P) at 1:5000, both diluted in 5% milk in 1x PBS with 0.1% Tween20.

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