MDA, GSH, SOD, GPx, and total protein were assessed using commercially available kits (catalog # 10009055, 703002, 706002, 703102 and 701780, respectively, Cayman® Chemical, Ann Arbor, MI, USA). Skin content of IL-6, TNF-α and hydroxyproline were determined using ELISA Kits (catalog # ab100772, ab236712 and ab222941, Abcam®, Cambridge, UK) as stated by the manufacturer’s instructions.

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