Wound tissues collected at days 7 and 14 were fixed in 10% formalin/saline for 24 h. This was followed by dehydration in serial concentrations of ethanol, cleared in xylene and embedded in paraffin. Tissues in paraffin block were sectioned (5 µm thickness). After dewaxing, tissues were rehydrated. Some sections were stained using hematoxylin and eosin (H&E) and the rest were stained with Masson’s trichrome. Histological examination was performed by an experienced pathologist without knowledge of treatment groups. Depending on the degree of re-epithelization, granulation tissue formation, collagen deposition, inflammatory cell infiltration and wound healing phase (I, II or III) at day 7, sections were given scores based on their abundance ranging from – to +++.

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