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All four bacterial DNA were isolated according to New England Biolabs, Lab-JOT protocols as described in [5,6,7,8,9].

Interactions of the Plasmid DNA from K12 and R4 strains with the analyzed lactones were described in details in the same way as that of coumarin derivatives, α-amidoamides, and 1,2-diarylethanols [5,6,7].

The analysis of antibiotics such as cloxacillin was carried out in the same way as that of α-amidoamides as described earlier in the literature [6].

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