Four-week-old C57BL/6 J male mice (n = 40) were sourced from Hunan SJA Laboratory Animal Co. Ltd. China, which were bred and housed in specific pathogen-free conditions with 12-h day and night light cycles at 22 ± 2 °C temperature and 55 ± 10% relative humidity. The normal-fat diet (NFD) was made up of 41% corn, 26% bran, 29% bean cake, 1% salt, 1% bone meal, 1% lysine, and 1% other. The high-fat diet (HFD) was made up of 1% cholesterol, 10% egg yolk, 10% lard, 0.2% sodium cholate, and 78.8% NFD. The proximate nutrient content of the NFD was as follows: crude protein, 18%; crude fat, 4%; crude fiber, 5%; calcium, 1.0–1.8%; and phosphorus, 0.6–1.2%. The proximate nutrient content of the HFD was as follows: crude protein, 17.6%; crude fat, 19.7%; crude fiber, 4%; calcium, 0.81–1.45%; and phosphorus, 0.56–1.04%. JSRS was provided by the Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China. The B. pseudolongum was provided by Inner Mongolia Agricultural University, China.

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