MPMs from OVX and sham female mice were cultured with the inoculum of S. aureus (1 x 106 CFU –MOI = 1:100) or sterile saline solution for 6 hours in 5% CO2 and 95% humid air at 37 °C. Part of MPMs from OVX females were pre-treated with 17β-estradiol (10-7 M) (Sigma Aldrich) for 24 hours at 37°C before stimulation with S. aureus. To assess whether sex would influence the response of macrophages to S. aureus, we performed the same experiment with MPMs derived from male mice.

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