An inoculum of S. aureus was prepared by direct suspension of 3 to 5 morphologically identical colonies of the reference strain into tubes containing sterile saline solution. The tubes were then vortexed and analyzed using a spectrophotometer to obtain an absorbance of 0.135 at 660 nm, equivalent to 1 × 108 colony-forming units (CFU) (de Oliveira et al., 2015). MPMs and HPBMs were subsequently inoculated at a multiplicity of infection (MOI) of 1:100.

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