We used an S. aureus strain isolated from a hospital in a previous study by our group, which has norA, mecA and blaZ genes, respectively, resistant to fluoroquinolone and beta-lactams (Carvalho et al., 2017). We used Brain Heart Infusion (BHI) and mannitol agar to activate, cultivate and sub-cultivate S. aureus strains for further inoculation in MPMs and HPBMs. In addition, Gram stain, catalase test, coagulase test and PCR (nuc gene) (Sasaki et al., 2010) were performed to confirm the identity and purity of the bacterial strain.

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