qPCR reactions were prepared with SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, Catalog #1725271) using cDNA and the following primers (final concentration, 0.05 μM): eF1a (Housekeeping) Fwd: 5’-CTACCTACCCTCCTCTTGGTCG-3’, Rev: 5’-CCTTAAGTAGAGTGCCCAGGT-3’; Rad21a Fwd: 5’-TACCTGCATAGTGAGATGTTCTGT-3’, Rev: 5’- ACAGAACAATGGAGGAAAAACAAC-3’; Rad21b Fwd: 5’-TATCCGTGCATGTGCATTTT-3’, Rev: 5’-CCTCTGGCTACATGATTTGC-3’; Rec8a Fwd: 5’-TGGTGAAGCCTATCCCTCCA-3’, Rev: 5’-CTTCTGGCTCTGGTGGTTGT-3’; Rec8b Fwd: 5’-AGATTCCCCCAAGCAAGTTCA-3’, Rev: 5’-ACAAACTGCATTTAAACTGACCTCT-3’. Cq values were determined for each primer pair and normalized to eF1a control. The fold change between mutant and wild-type was analyzed using the 2-ΔΔCt method. Raw Cq values and calculations are in S2 File.

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