Images of chromosome spreads and whole mount gonads stained with Ddx4 (Vasa) and DAPI were collected at the Department of Molecular and Cellular Biology Light Microscopy Imaging Facility at UC Davis. Chromosomes spreads were imaged using the Nikon N-SIM Super-Resolution microscope in 3D-SIM imaging mode with APO TIRF 100X oil lens. The images were collected and reconstructed using the NIS-Elements Imaging Software. Sections and fluorescent whole mounts were imaged using the Olympus FV1000 laser scanning confocal microscope. Images were processed using Fiji ImageJ software. Only linear modifications to brightness and contrast of whole images were applied. Images of eggs and embryos were acquired using a dissecting microscope. All data and related metadata underlying the findings have been deposited and are available at DRYAD (doi: 10.25338/B8V91Q) [81].

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