Euthanized fish were decapitated and cut open along the ventral midline to expose the body cavity. Alternatively, an additional cut was made at the anal fin to expose the body cavity if the fish was too small to make a ventral cut. The fish were fixed in 4% PFA in PBT at 4° C for 16–18 hours with gentle rocking. The fish were placed into fresh tubes and washed in 0.2% PBT 3 times for a minimum of 5 minutes each. Gonads were dissected out in PBT and placed into a ceramic 12-well plate, 1 gonad per well.

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