Spray-dried powder samples, 0.1; 0.5; and 1 g, were reconstituted in 100 mL Milli-Q water and adjusted to pH = 6.7. TPC of prepared samples were estimated using Folin–Ciocalteu method, as described by Kostić et al. [28]. Results were expressed as mg of GAE equivalents per 100 mL of sample (mg GAE/100 mL).

Antioxidant assays, such as total antioxidant capacity (in vitro phosphomolybdenum capacity) (TAC); ferric reducing power (FRP); ABTS•+ scavenging activity; and ferrous ion-chelating capacity (FCC), of prepared samples were evaluated as previously described by Pešić et al. [8] and Kostić et al. [28]. DPPH scavenging activity was evaluated using method developed by Oliveira et al. [29], with some modifications. Briefly, 105 μL of prepared samples were mixed with 840 μL of DPPH working solution and after incubation in the dark for 30 min, absorbance was measured at 515 nm (UV-1800, Shimadzu USA Manufacturing Inc., Canby, OR, USA). Obtained results for TAC, FRP, ABTS•+ and DPPH scavenging activity were expressed as µg of ascorbic acid equivalents per mL of sample (µg AAE/mL), while results for FCC was expressed as µg of EDTA equivalents per mL of sample (µg EDTA/mL).

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