Qualitative and quantitative analysis of PCs present in grape pomace seed extract were performed using an Accela Ultra high-performance liquid chromatography (UHPLC) system coupled to a linear ion trap-orbitrap mass spectrometer (LTQ OrbiTrap MS) equipped with a heated electrospray ionisation probe (HESI-II, ThermoFisher Scientific, Bremen, Germany) and Xcalibur software (version 2.1), as previously reported [6,8]. Quantification of all identified phenolic compounds was performed using available standards. The detection of phenolic compounds for which the standards were not been available was conducted based on their exact molecular masses and specific MS4 fragmentation. These compounds have been quantified and expressed as mg gallic acid; caffeic acid; catechin; quercetin–3-O-glucoside; or malvidin–3-O-glucoside per kg FW of seed, depending on the phenolic classes to which they belong.

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