The plasmid pEGFPN1-GalT (plasmid #11929) deposited by Jennifer Lippincott-Schwartz (Cole et al., 1996), pcDNA HA Arf1 DN T31 N (plasmid #10833) deposited by Thomas Roberts (Furman et al., 2002) and Arf1 T31N-EGFP (Arf1 DN) (plasmid #49580) deposited by Marci Scidmore were obtained from Addgene. The Arf1 WT-EGFP control plasmid was a gift from Isabelle Derré (Dept. of Microbiology, Immunology, and Cancer Biology, University of Virginia, VA, USA). Plasmids pECFP-C1-Rab1a WT, pECFP-C1-Rab1a DN (AS mutation), pECFP-C1-Rab2 WT, pECFP-C1-Rab2 DN, pECFP-C1-Rab6a WT, pECFP-C1 Rab6a DN, pECFP-C1-Rab11a WT and pECFP-C1-Rab11a DN were provided by W. D. H. Plasmid EGFP-CLASP1 DN overexpressing the microtubule-binding domain was a kind gift from Helder Maiato (Universidade do Porto, Portugal).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.