The plasmid pEGFPN1-GalT (plasmid #11929) deposited by Jennifer Lippincott-Schwartz (Cole et al., 1996), pcDNA HA Arf1 DN T31 N (plasmid #10833) deposited by Thomas Roberts (Furman et al., 2002) and Arf1 T31N-EGFP (Arf1 DN) (plasmid #49580) deposited by Marci Scidmore were obtained from Addgene. The Arf1 WT-EGFP control plasmid was a gift from Isabelle Derré (Dept. of Microbiology, Immunology, and Cancer Biology, University of Virginia, VA, USA). Plasmids pECFP-C1-Rab1a WT, pECFP-C1-Rab1a DN (AS mutation), pECFP-C1-Rab2 WT, pECFP-C1-Rab2 DN, pECFP-C1-Rab6a WT, pECFP-C1 Rab6a DN, pECFP-C1-Rab11a WT and pECFP-C1-Rab11a DN were provided by W. D. H. Plasmid EGFP-CLASP1 DN overexpressing the microtubule-binding domain was a kind gift from Helder Maiato (Universidade do Porto, Portugal).

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