At the end of the 28-day toxicity test, SD rats were fasted overnight and underwent general anesthesia with CO2. Necropsies were conducted carefully on all the rats, which either died or survived during experiments. Tissues like liver, spleen, heart, kidney (both), adrenal gland, lung, brain, pituitary gland, thymus, urinary bladder, stomach, intestine, testis, ovary, epididymis, uterus, prostate, seminal vesicle, trachea, esophagus, thyroid gland, salivary gland, skin, femur, and Harderian gland nerves were examined for the macroscopic morphology, and then removed quickly, washed in saline, weighed, and kept in 10% neutral buffered formalin (#0151S, BBC Biochemical, Seattle, WA). In addition, the relative weight of each organ (viscera/body ratio) was calculated based on the animal’s body weight according to the following formula: organ weight/body weight on sacrifice day × 100.

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