Crude insect cell lysates expressing either the N core domain or S ectodomain (see above) were separated on replica 10% SDS-PAGE gels and transferred to PVDF membranes according to standard protocols. Membranes were blocked with 1% milk powder and then probed with individual patient samples, diluted 1:50 in PBST containing 1% milk powder. Antigen-specific antibodies were detected by chemiluminescence using a rabbit anti-human IgG horseradish peroxidase conjugate; detected bands were compared to the expected molecular weight of the N and S proteins to confirm the specificity of detection. Antigen-specific IgG signals in individual patient samples were scored as strong, weak or absent by visual inspection of the western blots.

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