Identification of Differentially Expressed lncRNAs and mRNAs in MF vs. AMI and HF vs. MF

Differential expression analysis of lncRNAs and mRNAs in MF vs. AMI and HF vs. MF was evaluated in the R-bioconductor package DESeq2. Firstly, in order to normalize for sequencing depth, the original read count performed normalization. Then, the hypothesis testing probability (p-value) was obtained by the statistical model. Thirdly, multiple hypothesis test correction was carried out to obtain the corrected p-value [false discovery rate (FDR)]. Those differentially expressed lncRNAs and mRNAs were identified under the criterion of p-value < 0.05 and |log2Fold change| ≥ 1.

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