Pre-add 2.970 or 2.992 mL of freshly made E3 medium to each well, in accordance with the volume of compound that will be added. Mix compounds in the stock aliquot three times by pipetting up and down or by vortexing. Add 30 μl (for 100 μM solution) or 7.5 μl (for 25 μM solution) compound stock to each well and mix medium in wells four times to ensure homogenous distribution of the compound within the well. Transfer single larva with a glass pipette to each well in a minimum E3 volume for about twelve to fifteen larvae per well. Incubate the screening plate at 28°C covered with aluminum foil to protect compounds from light. For media changes, use the same procedure.

Recommendation:

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.