First weigh out 0.05 g cholesterol (Mw = 386.65 g/mol) and 0.45 g standard zebrafish larval food (SP; Sparos Lda; Zebrafeed <100 μm) and place in a 5 mL glass beaker to create a 10% w/w cholesterol-enriched diet, referred here as HCD. Components are homogenized and mixed by adding 1.5 mL diethyl ether that allows cholesterol solubilization. In another glass beaker, 1.5 mL diethyl ether is added to 0.5 g of SP to serve as a control diet (SPE; SP with diethyl ether). Diets are left overnight in the hood for the diethyl ether to evaporate completely and grounded up into fine particles using a pestle the following day.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.