For the high-cholesterol diet gut inflammation (HCD-GI) assay use 6 dpf larvae arising from group matings between heterozygous Tg(Lyz:NTR-mCherry)sh260 (Buchan et al., 2019). Collect embryos by natural spawning and raise them in polystyrene petri dishes at 28°C in E3 medium supplemented with 0.03% methylene blue as an antifungal agent. It is essential to maintain a density of embryos not exceeding 50 per petri dish. Great care should be taken to remove unfertilized eggs and chorions post hatching with a plastic Pasteur pipette under the stereoscope.

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