Statistical differences between groups were assessed using Student’s t-test (Prism 6.0, GraphPad, La Jolla, CA, USA). Statistical significance between differences was set at p < 0.05. Differences between reproductive output and egg yolk volume in 3 hpf embryos was assessed with t-tests between Casper and RedEfish. Similarly, the vitamin E contents of the embryos are reported as comparisons between the two fish lines. Additionally, tocopherol data were logarithmically transformed data for each fish line, then 2-factor ANOVA was followed by Holm–Sidak’s multiple comparisons test to allow for comparison between α- and γ-tocopherol concentrations in each fish line. Data are reported as means ± standard deviation.

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