2.6. Egg Quality and Embryo Morphological Assessment
This protocol is extracted from research article:
RedEfish: Generation of the Polycistronic mScarlet: GSG-T2A: Ttpa Zebrafish Line
Antioxidants (Basel), Jun 16, 2021; DOI: 10.3390/antiox10060965

RedEfish embryos were collected, staged, and incubated in standard EM. Egg traits were assessed by egg yolk diameter followed by egg volume estimated based on the volume of an ellipsoid using the equation [38,39]:

At 24 hpf and 5 dpf, RedEfish and Casper embryos were assessed for morbidity and mortality outcomes, as described [36,40]. RedEfish and Casper 5 dpf larval α- and γ-tocopherol concentrations were determined using high-performance liquid chromatography with electrochemical detection, as described with comparison to authentic standards [41].

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