Total RNA from the sham and TAC-operated hearts was extracted using ultrapure RNA kit (CWBio, Beijing, China). The obtained mRNA was reverse transcribed to cDNA using the PrimeScriptTM RT Master Mix (Dalian, China). Following this, each 384-well plate was mixed with 10 ng cDNA, 5 µL qPCR SYBR Mix (Yeason, China), 3 µL ddH2O, and 1 µL primer. The primer sequences were provided in Supplementary Table 5.

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