All experimental protocols and methods were carried out in accordance with the animal use and care protocol (# 5068) approved by the Institutional Animal Care and Use Committee at Oregon State University. 5D tropical strain and Tg zebrafish (Casper; mitfaw2/w2, mpv17a9/a9) strain (hereafter called Casper) [32] and c269 (Tg(UAS-E1b:NfsBmCherry)c264/+) strain (hereafter called c269) [33] were reared in Sinnhuber Aquatic Research Laboratory at Oregon State University under standard laboratory conditions of 28 °C on a 14 h light/10 h dark photoperiod according to standard zebrafish breeding protocols [34]. The 5D tropical strain and the c269Tg reporter line zebrafish were used as additional negative and positive controls for experiments, noted throughout.

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