All experimental protocols and methods were carried out in accordance with the animal use and care protocol (# 5068) approved by the Institutional Animal Care and Use Committee at Oregon State University. 5D tropical strain and Tg zebrafish (Casper; mitfaw2/w2, mpv17a9/a9) strain (hereafter called Casper) [32] and c269 (Tg(UAS-E1b:NfsBmCherry)c264/+) strain (hereafter called c269) [33] were reared in Sinnhuber Aquatic Research Laboratory at Oregon State University under standard laboratory conditions of 28 °C on a 14 h light/10 h dark photoperiod according to standard zebrafish breeding protocols [34]. The 5D tropical strain and the c269Tg reporter line zebrafish were used as additional negative and positive controls for experiments, noted throughout.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.