Analysis of CTs (CYN, ATX, NOD, dmMC-RR, MC-RR, MC-YR, MC-HtyR, dmMC-LR, MC-LR, MC-HilR, MC-WR, MC-LA, MC-LY, MC-LW, and MC-LF) and CPs (MG FR1, MG FR3, MG T1, MG T2, AER 602/K139, AER 298A, AEG A, AP B, AP F, and OSC Y) in water samples was carried out by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). For the determination of intra- and extracellular CTs and CPs, water samples were first filtered through GF/F filters and then the filters and filtered water were analyzed. Intracellular CTs and CPs were extracted from the filters’ biomass by an extraction mixture containing 75% MeOH:25% H2O. After evaporation of the extract and reconstitution with MeOH: H2O (5:95 v/v), the final solution was injected into the LC-MS/MS for analysis [31]. Filtered water samples were pre-treated using the dual cartridge (HLB and Envi-Carb) SPE process [33]. Briefly, water samples, after adjustment to pH 11, were passed through a dual cartridge assembly of HLB and ENVI-Carb. Recovery of extracellular CTs and CPs was achieved by reversing the cartridges and eluting with a mixture of 10 mL DCM:MeOH (40:60, v/v), containing 0.5% FA. The extract was dried and the residue was re-dissolved with 400 μL MeOH: H2O (5:95, v/v) prior to LC-MS/MS analysis.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.