Serum samples were collected from all the participants’ venous blood. Samples were centrifuged at 1,500 rpm for 15 min, then stored in -80°C for further analysis. The total RNA was extracted from the serum samples by using Tizol Reagent (BD Molecular Research Center, United States) according to the protocols of the manufacturers. In accordance with the instructions of the manufacturers, miRNA labeling and hybridization were conducted by using miRCURYTM Array Power Labeling kit (Exiqon, Denmark, 208032-A) and miRCURYTM Array Wash buffer kit (Exiqon, Denmark, 208021).

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