All calculations were performed using the Stata v15.0 software package (Stata Corporation). Categorical variables were presented as percentages and were compared by the χ 2 or Fischer exact test using an alpha of 0.05. Continuous variables were presented as means with SD and range and were compared by Student's t‐test. Univariate analysis was performed to study the association of the development and levels of different antibody isotypes with age and sex. Also, a multiple regression analysis was performed to examine the association of the age, sex, duration of symptoms, and disease severity with the IgG, IgM, and IgA antibody levels as determined by ELISA units. The Strengthening the Reporting of Observational Studies in Epidemiology guidelines were followed in the preparation of this manuscript.

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