The StarDist distribution applied here was version 0.6.0, downloaded from the author’s GitHub page [20]. Parameters used included a batch size of 2 and a training patch size of 128 × 128. The number of rays for the star distributed polygons was set at 32, the author recommended value. During prediction, the non-maximum suppression (nms) threshold was set to 0.3 and the probability threshold to 0.5. Additional settings were left to their default values, including binary cross-entropy training loss function and mean average error polygon distance loss function parameters.

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