2.9. Visualization of Autophagy Induced by GENI in Yeast

The YOM38 yeast cells containing the pR316-GFP-Atg8 plasmid yeast strain in glucose liquid medium with initial OD600 value of 0.1 were treated with 0, 0.3, 1, and 3 µM GENI or 30 µM RES for 22 h. Cells were washed thrice with PBS and stained with 4′,6-diamidino-2-phenylindole staining solution (20 µg/µL) in the dark. After 10 min, the yeast was washed thrice with PBS and observed using a vertical two-photon confocal fluorescence microscope (Olympus FV1000BX-51, Tokyo, Japan).

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