2.9. Visualization of Autophagy Induced by GENI in Yeast

The YOM38 yeast cells containing the pR316-GFP-Atg8 plasmid yeast strain in glucose liquid medium with initial OD600 value of 0.1 were treated with 0, 0.3, 1, and 3 µM GENI or 30 µM RES for 22 h. Cells were washed thrice with PBS and stained with 4′,6-diamidino-2-phenylindole staining solution (20 µg/µL) in the dark. After 10 min, the yeast was washed thrice with PBS and observed using a vertical two-photon confocal fluorescence microscope (Olympus FV1000BX-51, Tokyo, Japan).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.