2.7. Measurement of SOD, GPx, and CAT Enzymes Activities upon Treatment of GENI in Yeast

The SOD, GPx, and CAT enzymes activities were measured as described in our previous study [20]. The sample processing of BY4741 yeast and the protein extraction were the same as that of MDA measurement in Section 2.6. After determining the concentration of the protein, the activities of SOD, CAT, and GPx were determined using SOD (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), CAT, and GPx (Beyotime Biotechnology Limited Company, Shanghai, China) assay kits, respectively, in accordance with the manufacturer’s instructions. Detailed steps are shown in the Supporting Information (S2.2).

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