The antioxidative stress assay was performed as described in our previous study [20]. For the antioxidant qualitative test, the BY4741 yeast strain in glucose liquid medium with an initial 0.1 OD600 value was treated with GENI (0,1, and 3 µM) or RES (positive control, 10 µM) and cultured with shaking at 28 °C and 180 rpm for 24 h. From each group, 5 µL of yeast medium was inoculated in glucose solid medium with 9.5 mM H2O2 and cultured at 28 °C for 48 or 72 h. The growth of the yeast was observed and recorded.

For the antioxidant quantitative test, the BY4741 yeast strain in glucose liquid medium treated with samples was cultured with shaking at 28 °C and 160 rpm for 12 h. A blank control medium (0 mM H2O2 in glucose solid medium) and hydrogen peroxide group medium (5.5 mM H2O2 in glucose solid medium) were prepared, painted with 200 yeast cells of the different sample groups, and cultured at 28 °C for 48 h. The number of colonies in each group was counted, and the survival rate of each group (number of colonies in the hydrogen peroxide group/number of colonies in the blank control group × 100%) was calculated, plotted, and analyzed.

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