Immunohistochemical studies were performed to detect the expression of Bax, Bcl2, and caspase-3 proteins on tumor sections. Briefly, the sections were examined under a light microscope and photographed after dewaxing and hydration, antigen retrieval, blocking, incubation with primary and HRP-coupled secondary antibodies, diaminobenzidine (DAB) development, and hematoxylin counterstaining, respectively. The above reagents were purchased from Wuhan Sanying Biotechnology Company. Protein expression was quantified using Image J (National Institutes of Health, USA).

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