Three mice were randomly selected from each group, euthanized 24 hours after irradiation, and the tumor tissue was immediately excised and then fixed in paraformaldehyde for 24 hours. The tumor tissues were embedded in paraffin, sliced and dewaxed. Five sections of each tumor tissue were subjected to HE staining and image acquisition. Another five sections of each tumor tissue were tested to determine the number of apoptotic cells in the tumor tissue using the Apoptosis Detection Kit (Roche, Switzerland). Sections were observed with a microscope and photographed.

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