BM-MSCs at passage 3 were infected with lentiviral medium (GeneChem, Shanghai, China) containing enhanced green fluorescent protein (EGFP) or red fluorescent protein (RFP) according to the manufacturer’s instructions to obtain BM-MSCs expressing EGFP or RFP, which were called BM-MSCs-EGFP or BM-MSCs-RFP, respectively. Stably infected BM-MSCs were selected with 1.5 μg/ml puromycin (Solarbio, Beijing, China). BM-MSCs-EGFP were differentiated into IOBs, and BM-MSCs-RFP were differentiated into IECs for scaffold loading. SC nuclei were stained with 100 μg/ml Hoechst 33342 (Beyotime) to obtain labeled SCs.

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