BM-MSCs at passage 3 were infected with lentiviral medium (GeneChem, Shanghai, China) containing enhanced green fluorescent protein (EGFP) or red fluorescent protein (RFP) according to the manufacturer’s instructions to obtain BM-MSCs expressing EGFP or RFP, which were called BM-MSCs-EGFP or BM-MSCs-RFP, respectively. Stably infected BM-MSCs were selected with 1.5 μg/ml puromycin (Solarbio, Beijing, China). BM-MSCs-EGFP were differentiated into IOBs, and BM-MSCs-RFP were differentiated into IECs for scaffold loading. SC nuclei were stained with 100 μg/ml Hoechst 33342 (Beyotime) to obtain labeled SCs.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.