Orthotopic tumor-bearing mice were randomly assigned to six different treatment groups 7 days after tumor model establishment (n = 5/group): 1. PBS (150 μL), 2. BT (6 Gy), 3. RBC/CANS (2.5 mg/kg), 4. PLT/CANS (2.5 mg/kg), 5. RBC/CANS (2.5 mg/kg) + BT (6 Gy), and 6. PLT/CANS (2.5 mg/kg) + BT (6 Gy). All nanomaterials were intravenously injected into treated mice. Mice in groups 5 and 6 underwent irradiation at 6 h following nanoparticle injection. Before irradiation, mice were anesthetized using 4% isoflurane and an endoscope was used to visualize the tumor. The endoscopic probe was inserted from the right probe, while the BT implant probe was inserted from the left side. The irradiation was conducted via an implanted probe connected to an afterload system, which utilizes 192Ir as the irradiation source. Mice were monitored for up to 56 days, at which time all surviving mice were euthanized. In a second experiment, mice were assigned to the same treatment groups but were euthanized at 14 days post-treatment, at which time colon tissues were surgically excised and examined.

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